1524年，前刺客埃齐奥·奥迪托雷（Ezio Auditore）和他的妻子索非亚（Sofia）以及孩子马塞洛（Marcello）和弗拉维亚（Flavia）退居托斯卡纳乡村的别墅。 当埃齐奥（Ezio）在他的研究中写作时，索非亚（Sofia）要求他观看弗拉维亚（Flavia），而她和马塞洛（Marcello）拜访了他的妹妹克劳迪娅（Claudia）。 不久之后，当弗拉维亚（Vlavia）在别墅附近玩耍时，他离开书房去照管葡萄园。 在收获的过程中，他看到一个穿着黑色衣服的陌生人与弗拉维亚交谈。 埃拉·埃齐奥（Ezio）惧怕最坏情况，急忙拦截了这位陌生人，尽管弗拉维亚（Flavia）告诉他没有危险，因为埃齐奥（Ezio）看到她的项链上有刺客的徽章...
In the year 1524, the former Assassin Ezio Auditore retired to a villa in the countryside of Tuscany, with his wife Sofia and children Marcello and Flavia. While Ezio wrote in his study, Sofia asked him to watch Flavia while she and Marcello visit his sister Claudia. Shortly after, he left his study to tend their vineyards while Flavia played nearby the villa. In the midst of his harvest, he saw a stranger cloaked in black, talking to Flavia. Fearing the worst, Ezio hurriedly intercepted the stranger, though Flavia told him that she was no danger, as Ezio saw her necklace with the Assassin insignia...
